概述

  • 产品名称Anti-IL3RA抗体[6H6]
    参阅全部 IL3RA 一抗
  • 描述
    小鼠单克隆抗体[6H6] to IL3RA
  • 经测试应用适用于: ICC/IF, IP, Flow Cyt, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation. IL3R transfected COS cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号6H6
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab21562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. 100 µl volume or 100 µl of whole blood.



ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Detects a band of approximately 70 kDa.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能This is a receptor for interleukin-3.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD123 antibody
    • CD123 antigen antibody
    • hIL 3Ra antibody
    • hIL-3Ra antibody
    • hIL3Ra antibody
    • IL 3 receptor alpha SP2 isoform antibody
    • IL 3R alpha antibody
    • IL-3 receptor subunit alpha antibody
    • IL-3R subunit alpha antibody
    • IL-3R-alpha antibody
    • IL-3RA antibody
    • IL3 Receptor alpha antibody
    • IL3R antibody
    • Il3ra antibody
    • IL3RA_HUMAN antibody
    • IL3RAX antibody
    • IL3RAY antibody
    • IL3RX antibody
    • IL3RY antibody
    • Interleukin 3 receptor alpha chain antibody
    • Interleukin 3 receptor, alpha (low affinity) antibody
    • Interleukin 3 receptor, alpha antibody
    • Interleukin-3 Receptor alpha antibody
    • Interleukin-3 receptor subunit alpha antibody
    • Interleukin3 receptor antibody
    • Interleukin3 receptor, Y-chromosomal antibody
    see all

Anti-IL3RA antibody [6H6] 图像


  • Immunofluorescence analysis of Human peripheral blood mononuclear cells, staining IL3RA with ab21562.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) for 24 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.

    See Abreview

Anti-IL3RA antibody [6H6] (ab21562)参考文献

This product has been referenced in:
  • Herling M  et al. TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+ CD56+ blastic tumors of skin. Blood 101:5007-9 (2003). Read more (PubMed: 12576313) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (normal spleen)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH7.8
Permeabilization No
Specification normal spleen
Blocking step Casein as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative Formaldehyde
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Verified customer

提交于 Sep 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human head & neck squamous cell carcinoma)
Specification Human head & neck squamous cell carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 (0.1%) for 5 min
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
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Abcam user community

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提交于 Jan 30 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human peripheral blood mononuclear cells)
Specification Human peripheral blood mononuclear cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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提交于 Jan 28 2013

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse spleen whole extract)
Loading amount 100 µg
Specification Mouse spleen whole extract
Gel Running Conditions Reduced Denaturing (Tris-Glycine -10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 30 2012

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse spleen whole extract)
Loading amount 100 µg
Specification Mouse spleen whole extract
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Oct 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (PBMCs)
Specification PBMCs
Preparation Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
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提交于 Oct 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (tonsil)
Specification tonsil
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 25°C
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Abcam user community

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提交于 Sep 28 2012

Thank you for your enquiry and your interest in our products.

Generally, each antibody and batch should be titrated to determine the optimal working dilutions/concentrations.

ab17051: this antibody has only been tested in ICC/IF and...

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DISCOUNT CODE: *** Expiration date: January 30, 2012 Thanks for your call today and for your interest in ab21562. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab...

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Thank you for your enquiry. I have added a paper to the antibody datasheet that uses the 6H6 antibody to stain tissue sections. This paper has used paraformaldehyde as a fixative. It does not look like they used antigen retrieval (although I was u...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"