Synthetic peptide corresponding to Human IL36 gamma aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q9NZH8
Our Abpromise guarantee covers the use of ab116281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116281 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab116281 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"