概述
-
产品名称Anti-IL36 gamma抗体
参阅全部 IL36 gamma 一抗 -
描述兔多克隆抗体to IL36 gamma
-
宿主Rabbit
-
经测试应用适用于: WBmore details
-
种属反应性与反应: Human
预测可用于: Chimpanzee, Gorilla
-
免疫原
Synthetic peptide corresponding to Human IL36 gamma aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: Q9NZH8 -
阳性对照
- This antibody gave a positive signal in Human Tonsil tissue lysate.
性能
-
形式Liquid
-
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
-
克隆多克隆
-
同种型IgG
-
研究领域
相关产品
-
Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab116281 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
靶标
-
功能Function as an agonist of NF-kappa B activation through the orphan IL-1-receptor-related protein 2. Could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1), that is present in epithelial barriers and takes part in local inflammatory response.
-
组织特异性Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus. In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Up-regulated in lesional psoriasis skin.
-
序列相似性Belongs to the IL-1 family.
-
细胞定位Secreted.
- Information by UniProt
-
数据库链接
- Entrez Gene: 56300 Human
- Omim: 605542 Human
- SwissProt: Q9NZH8 Human
- Unigene: 211238 Human
-
别名
- IL 1 epsilon antibody
- IL 1 related protein 2 antibody
- IL 1(EPSILON) antibody
see all
图片
-
Western blot - Anti-IL36 gamma antibody (ab116281)Anti-IL36 gamma antibody (ab116281) at 1 µg/ml + Tonsil (Human) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
Additional bands at: 90 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116281 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
文献
ab116281 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"