概述

  • 产品名称Anti-IL36 beta抗体
    参阅全部 IL36 beta 一抗
  • 描述
    兔多克隆抗体to IL36 beta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to Human IL36 beta aa 50-150 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9NZH7

  • 阳性对照
    • This antibody gave a positive signal in Human Tonsil tissue lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 19 kDa).

靶标

  • 组织特异性Expression at low levels in tonsil, bone marrow, heart, placenta, lung, testis and colon but not in other tissues or in any hematopoietic cell lines.
  • 序列相似性Belongs to the IL-1 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2310043N20Rik antibody
    • Family of interleukin 1 eta antibody
    • FIL1 (ETA) antibody
    • FIL1 antibody
    • FIL1 eta antibody
    • FIL1H antibody
    • FILI-(ETA) antibody
    • IL 1F8 (FIL1 eta antibody
    • IL 1F8 antibody
    • IL 1H2 antibody
    • IL-1 eta antibody
    • IL-1F8 antibody
    • IL-1H2 antibody
    • IL1 ETA antibody
    • IL1F8 (Canonical product IL 1F8a) antibody
    • IL1F8 antibody
    • IL1F8_HUMAN antibody
    • IL1H2 antibody
    • IL36B antibody
    • Interleukin 1 family, member 8 (eta) antibody
    • Interleukin 1 family, member 8 antibody
    • Interleukin 1 homolog 2 antibody
    • Interleukin 1 Superfamily e antibody
    • Interleukin 36 beta antibody
    • Interleukin-1 eta antibody
    • Interleukin-1 family member 8 antibody
    • Interleukin-1 homolog 2 antibody
    • MGC126880 antibody
    • MGC126882 antibody
    • OTTMUSP00000012797 antibody
    • RP23-176J12.1 antibody
    see all

Anti-IL36 beta antibody 图像

  • All lanes : Anti-IL36 beta antibody (ab116280) at 1 µg/ml (Milk blocking 3%)

    Lane 1 : Tonsil (Human) Tissue Lysate
    Lane 2 : Tonsil (Human) Tissue Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Donkey Anti-Rabbit IgG H&L preadsorbed (ab97081) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 19 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa (possible non-specific binding).

    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab116280 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL36 beta antibody (ab116280)参考文献

ab116280 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab116280.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"