Anti-IL36 alpha抗体(ab116279)

概述

  • 产品名称Anti-IL36 alpha抗体
    参阅全部 IL36 alpha 一抗
  • 描述
    兔多克隆抗体to IL36 alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human IL36 alpha aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9UHA7

  • 阳性对照
    • This antibody gave a positive signal in Human Adult and Fetal Brain tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).

靶标

  • 组织特异性Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.
  • 序列相似性Belongs to the IL-1 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FIL1 antibody
    • FIL1 epsilon antibody
    • FIL1(EPSILON) antibody
    • FIL1E antibody
    • IL 1 epsilon antibody
    • IL 1F6 antibody
    • IL 1H1 antibody
    • IL-1 epsilon antibody
    • IL-1F6 antibody
    • IL1E antibody
    • IL1F6 antibody
    • IL1F6_HUMAN antibody
    • IL1RP2 antibody
    • IL36A antibody
    • Interleukin 1 epsilon antibody
    • Interleukin 1 family member 6 (epsilon) antibody
    • Interleukin 1 family member 6 antibody
    • Interleukin 36 alpha antibody
    • Interleukin-1 epsilon antibody
    • Interleukin-1 family member 6 antibody
    • MGC129552 antibody
    • MGC129553 antibody
    • MGC151479 antibody
    • MGC151481 antibody
    • OTTMUSP00000012798 antibody
    • RP23-176J12.4 antibody
    see all

Anti-IL36 alpha antibody 图像

  • All lanes : Anti-IL36 alpha antibody (ab116279) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue
    Lane 3 : Brain (Human) Tissue Lysate - adult normal tissue with Immunising peptide at 1 µg/ml
    Lane 4 : Brain (Human) Tissue Lysate - fetal normal tissue with Immunising peptide at 1 µg/ml

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab116279 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL36 alpha antibody (ab116279)参考文献

ab116279 has not yet been referenced specifically in any publications.

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