Anti-IL33抗体(ab110535)

概述

  • 产品名称Anti-IL33抗体
    参阅全部 IL33 一抗
  • 描述
    兔多克隆抗体to IL33
  • 经测试应用适用于: Neutralising, WB, Indirect ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Recombinant Fragment
    预测可用于: Human
  • 免疫原

    Highly pure (>98%) recombinant Human IL33.

性能

  • 形式Lyophilised:Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.20
    Constituent: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab110535 was purified by affinity chromatography employing immobilized hIL33 matrix.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab110535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use a concentration of 0.2 - 0.4 µg/ml. To yield one-half maximal inhibition [ND50] of the biological activity of Human IL33 (1.0 ng/ml).
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 30 kDa. The detection limit for recombinant hIL33 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. ab110535, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL33 (Biotin) (ab84249). ab110535, in conjunction with ab84249 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL33.

靶标

  • 功能Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
  • 组织特异性Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • 序列相似性Belongs to the IL-1 family. Highly divergent.
  • 结构域The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • 翻译后修饰The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
  • 细胞定位Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt
  • 数据库链接
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Anti-IL33 antibody (ab110535)参考文献

ab110535 has not yet been referenced specifically in any publications.

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Thank you for your enquiry and your interest in our products. I can confirm that this particular antibody (ab110535 - Anti-IL33 antibody) has been tested and characterized in neutralizing assay. As the datasheet indicates in order to yield one-h...

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