概述

  • 产品名称Anti-IL33抗体[4E9]
    参阅全部 IL33 一抗
  • 描述
    小鼠单克隆抗体[4E9] to IL33
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human IL33 (112-270 aa) purified from E. coli

  • 阳性对照
  • 常规说明


    ab72844 is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL33 protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab72844 was purified from mouse ascitic fluids by protein G affinity chromatography.
  • Primary antibody说明ab72844 is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human IL33 protein.
  • 克隆单克隆
  • 克隆编号4E9
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab72844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Detects a band of approximately 26 kDa (predicted molecular weight: 31 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 功能Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
    In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100).
  • 组织特异性Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.
  • 序列相似性Belongs to the IL-1 family. Highly divergent.
  • 结构域The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association.
  • 翻译后修饰The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.
  • 细胞定位Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418).
  • Information by UniProt
  • 数据库链接
  • 别名
    • C9orf26 antibody
    • CHROMOSOME 9 OPEN READING FRAME 26 antibody
    • DKFZp586H0523 antibody
    • DVS27 antibody
    • DVS27 related protein antibody
    • IL 1F11 antibody
    • IL 33 antibody
    • IL-1F11 antibody
    • IL-33 antibody
    • IL1F11 antibody
    • IL33 antibody
    • IL33_HUMAN antibody
    • Interleukin 1 family member 11 antibody
    • Interleukin 33 antibody
    • INTERLEUKIN 33 NFHEV antibody
    • Interleukin 33 precursor antibody
    • Interleukin-1 family member 11 antibody
    • Interleukin-33 (109-270) antibody
    • Interleukin33 antibody
    • NF HEV antibody
    • NF-HEV antibody
    • NFEHEV antibody
    • NFHEV antibody
    • Nuclear factor for high endothelial venules antibody
    • Nuclear factor from high endothelial venules antibody
    • OTTHUMP00000021041 antibody
    • RP11 575C20.2 antibody
    see all

Anti-IL33 antibody [4E9] 图像

  • Anti-IL33 antibody [4E9] (ab72844) at 1/500 dilution + Jurkat cell lysate at 35 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 31 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)

Anti-IL33 antibody [4E9] (ab72844)参考文献

This product has been referenced in:
  • Wang Y  et al. Exposure to cigarette smoke impacts myeloid-derived regulatory cell function and exacerbates airway hyper-responsiveness. Lab Invest 94:1312-25 (2014). WB ; Mouse . Read more (PubMed: 25365203) »

See 1 Publication for this product

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