Anti-IL-32抗体(ab37158)
Key features and details
- Rabbit polyclonal to IL-32
- Suitable for: IHC-P, WB, ICC/IF, ELISA
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-IL-32抗体
参阅全部 IL-32 一抗 -
描述
兔多克隆抗体to IL-32 -
宿主
Rabbit -
特异性
ab37158 recognises all 4 isoforms of IL-32 (100% sequence alignment to each). -
经测试应用
适用于: IHC-P, WB, ICC/IF, ELISAmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IL-32 (C terminal).
(Peptide available asab39778) -
阳性对照
- WB: Human spleen lysates. IHC-P: Human spleen tissue. ICC/IF: Human spleen cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37158于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 10 µg/ml.
|
WB |
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights).
|
|
ICC/IF |
Use a concentration of 20 µg/ml.
|
|
ELISA |
Use at an assay dependent concentration. PubMed: 18544573
|
说明 |
---|
IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). All 4 isoforms of IL32 can be detected by this antibody. The larger precursor can be detected at approximately 26kDa. A smaller band can sometimes be observed at approximately 12.5kDa. This consists of multiple isoforms (this can appear smeared as they are slightly different molecular weights). |
ICC/IF
Use a concentration of 20 µg/ml. |
ELISA
Use at an assay dependent concentration. PubMed: 18544573 |
靶标
-
功能
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. -
组织特异性
Selectively expressed in lymphocytes. Expression more prominent in immunce cells than in non-immune cells. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 9235 Human
- Omim: 606001 Human
- SwissProt: P24001 Human
- Unigene: 943 Human
-
别名
- IL 32alpha antibody
- IL 32beta antibody
- IL 32delta antibody
see all
图片
-
Lane 1 : Anti-IL-32 antibody (ab37158) at 2 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 4 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 26 kDa -
Lane 1 : Anti-IL-32 antibody (ab37158) at 5 µg/ml
Lane 2 : Anti-IL-32 antibody (ab37158) at 10 µg/ml
All lanes : Human spleen cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 26 kDa
Observed band size: 12,26 kDa why is the actual band size different from the predicted?
Two isoforms of IL32 can be seen -
IHC-P of Human spleen tissue with ab37158 at 10 µg/ml
-
Immunocytochemistry of IL-32 in Human Spleen cells using ab37158 at 20 ug/ml.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab37158 被引用在 12 文献中.
- Zhang J et al. IL-32 exacerbates adenoid hypertrophy via activating NLRP3-mediated cell pyroptosis, which promotes inflammation. Mol Med Rep 23:N/A (2021). PubMed: 33495843
- Liu D et al. Placenta-derived IL-32ß activates neutrophils to promote preeclampsia development. Cell Mol Immunol 18:979-991 (2021). PubMed: 33707686
- Bengts S et al. Altered IL-32 Signaling in Abdominal Aortic Aneurysm. J Vasc Res 57:236-244 (2020). PubMed: 32434199
- Dali-Youcef N et al. Interleukin-32 Contributes to Human Nonalcoholic Fatty Liver Disease and Insulin Resistance. Hepatol Commun 3:1205-1220 (2019). PubMed: 31497742
- Pavlovic M et al. Diverse Expression of IL-32 in Diffuse and Intestinal Types of Gastric Cancer. Gastroenterol Res Pract 2018:6578273 (2018). PubMed: 30402092