概述

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE.

靶标

Anti-IL31 antibody 图像

  • Lane 1 : Anti-IL31 antibody (ab62579) at 2.5 µg/ml
    Lane 2 : Anti-IL31 antibody (ab62579) at 5 µg/ml
    Lane 3 : Anti-IL31 antibody (ab62579) at 10 µg/ml

    Lane 1 : human skeletal muscle tissue lysate
    Lane 2 : human skeletal muscle tissue lysate
    Lane 3 : human skeletal muscle tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of IL-31 in Human Skeletal Muscle cells using ab62579 at 20 ug/ml.

Anti-IL31 antibody (ab62579)参考文献

ab62579 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue (Skin tissue)
Permeabilization No
Specification Skin tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
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提交于 Sep 23 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Tonsil)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization Yes - 0.1% Tween 20
Specification Tonsil
Blocking step BioGenex Power Block 10 mins then BioGenex NGS 30 mins as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative Formaldehyde
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提交于 Sep 14 2015

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Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Cell (Keratinocytes)
Specification Keratinocytes
Permeabilization Yes - 0,1%Saponin/PBS
Fixative Methanol
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提交于 Jul 17 2013

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Application Western blot
Sample Human Tissue lysate - whole (skin)
Loading amount 20 µg
Specification skin
Gel Running Conditions Reduced Denaturing (5-16% gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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提交于 Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"