Anti-IL-31抗体(ab62579)
Key features and details
- Rabbit polyclonal to IL-31
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-IL-31抗体
参阅全部 IL-31 一抗 -
描述
兔多克隆抗体to IL-31 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IL-31 (internal sequence).
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阳性对照
- Human skeletal muscle tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62579于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use a concentration of 20 µg/ml.
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WB | (1) |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE.
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说明 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 18 kDa). Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE. |
靶标
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功能
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
组织特异性
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 386653 Human
- Omim: 609509 Human
- SwissProt: Q6EBC2 Human
- Unigene: 569071 Human
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形式
Amino acids 1-23 are a signal peptide. -
别名
- IL 31 antibody
- IL-31 antibody
- IL31 antibody
see all
图片
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Lane 1 : Anti-IL-31 antibody (ab62579) at 2.5 µg/ml
Lane 2 : Anti-IL-31 antibody (ab62579) at 5 µg/ml
Lane 3 : Anti-IL-31 antibody (ab62579) at 10 µg/ml
All lanes : human skeletal muscle tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Despite its predicted size, IL31 runs at approximately 27 - 30 kDa in SDS-PAGE. -
Immunofluorescence of IL-31 in Human Skeletal Muscle cells using ab62579 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62579 尚未被引用在任何文献中。