概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL3
  • 经测试应用
    适用于: WB, ELISA, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hIL-3 (human Interleukin-3)

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Neutralising
  • 应用说明
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (2.0 ng/ml), a concentration of 0.06 - 0.1 µg/ml of this antibody is required.

    ELISA:To detect hIL-3 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-3.

    Western Blot:To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • 靶标

    • 功能
      Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
      This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
    • 组织特异性
      Activated T-cells, mast cells, natural killer cells.
    • 序列相似性
      Belongs to the IL-3 family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Colony stimulating factor multiple antibody
      • Hematopoietic growth factor antibody
      • IL 3 antibody
      • IL-3 antibody
      • IL3 antibody
      • IL3_HUMAN antibody
      • Interleukin 3 (colony stimulating factor, multiple) antibody
      • Interleukin 3 antibody
      • Interleukin-3 antibody
      • Mast cell growth factor antibody
      • MCGF antibody
      • MGC79398 antibody
      • MGC79399 antibody
      • Multi CSF antibody
      • Multilineage colony stimulating factor antibody
      • Multipotential colony stimulating factor antibody
      • Multipotential colony-stimulating factor antibody
      • OTTHUMP00000065963 antibody
      • P cell stimulating factor antibody
      • P-cell-stimulating factor antibody
      see all

    实验方案

    Anti-IL3 antibody (ab9620)参考文献

    ab9620 has not yet been referenced specifically in any publications.

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