Anti-IL27抗体[B-G49] (FITC) (ab84472)

概述

  • 产品名称Anti-IL27抗体[B-G49] (FITC)
    参阅全部 IL27 一抗
  • 描述
    小鼠单克隆抗体[B-G49] to IL27 (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 特异性Recognizes both natural and recombinant IL27.
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human IL27

  • 阳性对照
    • Dendritic cells.

性能

应用

Our Abpromise guarantee covers the use of ab84472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use 10µl for 106 cells or 100 µl of whole blood.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL-27 subunit p28 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replivation.
    • 组织特异性Expressed in monocytes and in placenta.
    • 序列相似性Belongs to the IL-6 superfamily.
    • 翻译后修饰O-glycosylated.
    • 细胞定位Secreted. Does not seem to be secreted without coexpression of EBI3.
    • Information by UniProt
    • 数据库链接
    • 别名
      • EBI3 antibody
      • IL 27 antibody
      • IL 27 p28 subunit antibody
      • IL 27A antibody
      • IL 30 antibody
      • IL-27 subunit alpha antibody
      • IL-27-A antibody
      • IL27 antibody
      • IL27-A antibody
      • IL27A antibody
      • IL27A_HUMAN antibody
      • IL27p28 antibody
      • IL30 antibody
      • Interleukin 27 antibody
      • Interleukin 27 subunit alpha antibody
      • Interleukin 27, 28-KD subunit antibody
      • Interleukin 30 antibody
      • Interleukin-27 subunit alpha antibody
      • p28 antibody
      see all

    Anti-IL27 antibody [B-G49] (FITC) 图像

    • Dendritic cells were activated with LPS 1µg/ml, IFN gamma 10ng/ml and TNF-alpha 100ng/ml for 48hours. Unactivated dendritic cells were used as negative control. 3.1 x 105 dendritic cells (activated = green line or resting cells = black line) were labelled with 10µl of ab84472.

    Anti-IL27 antibody [B-G49] (FITC) (ab84472)参考文献

    ab84472 has not yet been referenced specifically in any publications.

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