The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 24 kDa.
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. After incubation with the primary antibody, slides are incubated with biotinylated rabbit anti-goat IgG secondary antibody followed by alkaline phosphatase-streptavidin and chromogen.
Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.
Up-regulated in melanoma cells induced to terminally differentiate.
Margaryan NV et al. Melanocytes Affect Nodal Expression and Signaling in Melanoma Cells: A Lesson from Pediatric Large Congenital Melanocytic Nevi. Int J Mol Sci17:418 (2016).
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