Anti-IL23 Receptor抗体- Aminoterminal end (ab53656)

概述

  • 产品名称Anti-IL23 Receptor抗体- Aminoterminal end
    参阅全部 IL23 Receptor 一抗
  • 描述
    山羊多克隆抗体to IL23 Receptor - Aminoterminal end
  • 经测试应用适用于: WB, ICC, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Pig
  • 免疫原

    Synthetic peptide:

    CQPRKLHFYKNGIKERFQITRINKTTARL

    , corresponding to N terminal amino acids 59-87 of Human IL23 Receptor Note: Peptide sequence is <50% identical to other receptors in this region.

  • 阳性对照
    • Human and Mouse spleen paraffin sections.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab53656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 72 kDa.
ICC 1/400.
IHC-P 1/250.

靶标

  • 功能Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.
  • 组织特异性Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.
  • 疾病相关Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17) [MIM:612261]. IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
    Genetic variations in IL23R are a cause of susceptibility to psoriasis type 7 (PSORS7) [MIM:605606]. Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 2 fibronectin type-III domains.
  • 翻译后修饰Phosphorylated in response to IL23.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 23R antibody
    • IL-23 receptor antibody
    • IL-23R antibody
    • Il23r antibody
    • IL23R_HUMAN antibody
    • Interleukin 23 receptor antibody
    • Interleukin-23 receptor antibody
    see all

Anti-IL23 Receptor antibody - Aminoterminal end 图像

  • ab53656 at 1/250 dilution staining IL23 Receptor in human spleen by Immunohistochemistry, Paraffin embedded tissue.
  • ab53656 at 1/250 dilution staining IL23 Receptor in mouse spleen by Immunohistochemistry, Paraffin embedded tissue.
  • ab53656 staining IL23 in human colon by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formaldehyde fixed prior to blocking in 10% serum for 2 hours at 21°C. The primary antibody was diluted 1/200 and incubated with the sample for 2 hours at 21°C. Alexa fluor® 594 rabbit polyclonal was used as the secondary.

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Anti-IL23 Receptor antibody - Aminoterminal end (ab53656)参考文献

This product has been referenced in:
  • Li H  et al. IL-23 promotes TCR-mediated negative selection of thymocytes through the upregulation of IL-23 receptor and ROR?t. Nat Commun 5:4259 (2014). Read more (PubMed: 25001511) »
  • Helbling M  et al. Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients. Oncotarget 5:4671-82 (2014). Read more (PubMed: 25015728) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (lymph node)
Specification lymph node
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01 M citrate buffer
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Claudia Zizzadoro

Verified customer

提交于 Sep 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Retrieval Solution
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Username

Mrs. Nicole Schechter

Verified customer

提交于 Nov 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"