Anti-IL23抗体[B-Z23] (ab84471)


  • 产品名称Anti-IL23抗体[B-Z23]
    参阅全部 IL23 一抗
  • 描述
    小鼠单克隆抗体[B-Z23] to IL23
  • 特异性Recognizes both recombinant and natural human IL23, p19 sub-unit.
  • 经测试应用适用于: Neutralising, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human IL23 (sub-unit p19)


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Ion Exchange Chromatography
  • 纯化说明Sterile-filtered through 0.22 µm and treated to remove endotoxins.
  • 克隆单克隆
  • 克隆编号B-Z23
  • 骨髓瘤x63-Ag8.653
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab84471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Neutralising Use at an assay dependent dilution.
ICC/IF Use at an assay dependent concentration.


  • 功能Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 组织特异性Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 序列相似性Belongs to the IL-6 superfamily.
  • 发展阶段Expressed by newborns dendritic cells.
  • 细胞定位Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

Anti-IL23 antibody [B-Z23] 图像

  • ab84472 used in Neutralisation.
    A = untreated
    B = treated with IL-2 (10ng/ml) + IL-23 (10ng)
    C = treated with IL-2 (10ng/ml) + IL-23 (50ng/ml)
    D = treated with anti-IL-23 (5µg/ml) + IL-2 + IL-23 (50ng/ml)
    Human monocytes were fixed in methanol, permeabilized using 0.1% Saponin/ PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab84471 at 5µg/ml for 48 hours at 37°C.

    Staining: IL-17 (red) GFP (green).

    We have examined the effect of IL-23 and in combination with anti-IL-23 on IL-17 production in monocytes. IL-23 leads to increased IL-17 secretion (Figure B and C) and this effect was abolished with anti-IL-23 (Figure D). IL-2 is part of our culture medium for monocytes. IL-2 stimulation serves as a basis to keep the cell alive. Thus almost every cytokine that we used is used in combination with IL-2. Because it is part of the medium, IL-2 can be omitted when specifying the stimulation. The cells were stained with the GFP (Green Fluorescent Protein), just to make them visible. We opted for GFP, because the cells are less affected by GFP as by dyes.The red color represents the IL-17 which was the target in this experiment. The secretion of IL-17 was tested with and without IL-23 and in combination with anti-IL-23. If the green of the GFP interferes, all images can be shown without the green channel (red only).

    See Abreview

Anti-IL23 antibody [B-Z23] (ab84471)参考文献

ab84471 has not yet been referenced specifically in any publications.

Product Wall

The lab has provide me with the neutralization protocol used by them. The peptides and reagents are all listed on this. The test is done on murine cells for human cytokine, the mIL-23 and hIL-23 are closed and cross-reacted on cells from mice or human....

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Thank you for contacting us.

The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more spe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Monocytes)
Specification Monocytes
Fixative Methanol
Permeabilization Yes - 0,1% Saponin/PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

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Verified customer

提交于 Feb 15 2012

Thank you for contacting us. I have attached the protocol that we received from the laboratory that tested this IL23 antibody for neutralization. The assay measures the amount of IL17 that is produced by mouse splenocytes in response to IL23 and IL2...

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