概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL23
  • 特异性
    This antibody recognizes IL23A/IL-23 p19.
  • 经测试应用
    适用于: WB, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 16 amino acid synthetic peptide conjugated to KLH, corresponding to the C-terminus of Human IL23.

  • 阳性对照
    • Human Kidney, Mouse brain, Mouse kidney and Mouse liver tissues

性能

应用

Our Abpromise guarantee covers the use of ab115759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

靶标

  • 功能
    Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 组织特异性
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 序列相似性
    Belongs to the IL-6 superfamily.
  • 发展阶段
    Expressed by newborns dendritic cells.
  • 细胞定位
    Secreted. Secreted upon association with IL12B.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 23 A antibody
    • IL 23 antibody
    • IL 23 subunit alpha antibody
    • IL 23A antibody
    • IL 23p19 antibody
    • IL-23 subunit alpha antibody
    • IL-23-A antibody
    • IL-23p19 antibody
    • IL12B antibody
    • IL23 antibody
    • Il23a antibody
    • IL23A_HUMAN antibody
    • IL23P19 antibody
    • interleukin 12B antibody
    • Interleukin 23 alpha subunit p19 antibody
    • Interleukin 23 p19 subunit antibody
    • interleukin 23 subunit alpha antibody
    • interleukin 23 subunit p19 antibody
    • interleukin six, G CSF related factor antibody
    • Interleukin-23 subunit alpha antibody
    • Interleukin-23 subunit p19 antibody
    • JKA3 induced upon T cell activation antibody
    • MGC79388 antibody
    • P19 antibody
    • SGRF antibody
    see all

图片

  • All lanes : Anti-IL23 antibody (ab115759) at 1 µg/ml

    Lane 1 : Raji cell lysate
    Lane 2 : Raji cell lysate


    Predicted band size : 21 kDa

    Western blot of IL-23 in Raji cell lysate with ab115759 at 1 ug/ml in the (1) absence and (2) presence of blocking peptide.

  • Human Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Brain (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Kidney (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Mouse Liver (formalin-fixed, paraffin-embedded) stained with ab115759 at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

文献

This product has been referenced in:
  • Jiang H  et al. Interleukin-23 may contribute to the pathogenesis of lumbar disc herniation through the IL-23/IL-17 pathway. J Orthop Surg Res 11:12 (2016). IHC ; Human . Read more (PubMed: 26774625) »
  • Zhang HJ  et al. Effects of curcumin on interleukin-23 and interleukin-17 expression in rat retina after retinal ischemia-reperfusion injury. Int J Clin Exp Pathol 8:9223-31 (2015). Read more (PubMed: 26464670) »

See all 4 Publications for this product

客户评价及客户问答

Vielen Dank für Ihre Anfrage.

Ich kann Ihnen ab115759 (Anti-IL23 antibody) empfehlen.

http://www.abcam.com/index.html?datasheet=115759 (or use the following: http://www.abcam.com/index.html?datasheet=115759).

Dieser A...

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Thank you for contacting us.

The bovine protein is 81% similar to human protein so the possibility of cross reactivity is high. Unfortunately for monoclonal antibodies we do not know the exact epitope sequence so I am sorry I can't be more spe...

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Thank you for contacting us.

The C-terminal sequence of IL23A protein is quite homologous between Bovina and Human. http://www.uniprot.org/blast/uniprot/2012102343Z34R5CGA?alignment=25

It seems that the antibody e.g. ab115759; http://...

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Vielen Dank für Ihren Anruf.

Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

/Ich habe unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Frageboge...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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