概述

  • 产品名称Anti-IL22 Receptor Alpha抗体
    参阅全部 IL22 Receptor Alpha 一抗
  • 描述
    兔多克隆抗体to IL22 Receptor Alpha
  • 经测试应用适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    QSSNFENILTWDSGPE

    , corresponding to amino acids 31-46 of Human IL-22 Receptor.

  • 阳性对照
    • HepG2 whole cell lysate.
  • 常规说明Stable for one year.

性能

应用

Our Abpromise guarantee covers the use of ab5984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.Can be blocked with IL22 Receptor Alpha peptide (ab6229).
IHC-P Use a concentration of 5 µg/ml. PubMed: 19535621
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

Anti-IL22 Receptor Alpha antibody 图像

  • Immunohistochemistry of IL-22R in rat kidney tissue with IL-22R antibody at 5 µg/ml.

  • Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml. Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml.
  • Immunocytochemistry of IL-22R in HepG2 cells with IL-22R antibody at 10 µg/ml.
  • Immunofluorescence of IL-22 receptor in HepG2 cells using ab5984 at 10 ug/ml.

Anti-IL22 Receptor Alpha antibody (ab5984)参考文献

This product has been referenced in:
  • Andoh A  et al. Expression of IL-24, an activator of the JAK1/STAT3/SOCS3 cascade, is enhanced in inflammatory bowel disease. J Immunol 183:687-95 (2009). IHC-P ; Human . Read more (PubMed: 19535621) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Lung)
Gel Running Conditions Reduced Denaturing (12% Bis-Tris NuPAGE)
Loading amount 40 µg
Specification Lung
Blocking step Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Pete Serbedzija

Verified customer

提交于 Jul 05 2016

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (Lung)
Specification Lung
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Mar 25 2014

Application Flow Cytometry
Fixation Surface staining
Permeabilization No
Sample Human Cell (Epidermal keratinocytes)
Specification Epidermal keratinocytes
Gating Strategy Human epidermal keratinocytes
Preparation Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: Biolegend cell staining buffer
Username

Abcam user community

Verified customer

提交于 Feb 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"