概述

  • 产品名称Anti-IL21抗体
    参阅全部 IL21 一抗
  • 描述
    兔多克隆抗体to IL21
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    TCPSCDSYEKKPPKE

    , corresponding to amino acids 121-135 of Human IL-21.

  • 阳性对照
  • 常规说明Stable for one year.


    A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • Primary antibody说明A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab5978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.Can be blocked with IL21 peptide (ab6680).

靶标

  • 功能Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • 组织特异性Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • 序列相似性Belongs to the IL-15/IL-21 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CVID11 antibody
    • IL 21 antibody
    • IL-21 antibody
    • Il21 antibody
    • IL21_HUMAN antibody
    • Interleukin 21 antibody
    • Interleukin-21 antibody
    • interleukin-21 isoform antibody
    • Interleukin21 antibody
    • OTTHUMP00000164088 antibody
    • Za11 antibody
    see all

Anti-IL21 antibody 图像

  • ab5978 staining IL21 in mouse skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/8000 in 10% normal goat serum) for 10 hours. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/350) was used as the secondary antibody.

    See Abreview

  • Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml.

  • Developed using the ECL technique

    Performed under reducing conditions.
  • ab5978 at 2ug/ml staining of IL21 in K562 cells.

Anti-IL21 antibody (ab5978)参考文献

ab5978 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Mouse Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Ms. Hongyan Zhao

Verified customer

提交于 Feb 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 60 µg
Specification 293T
Treatment transfection of plasmid expressing IL21
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"