The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.15 - 0.30 µg/ml to detect at least 0.2 ng/well of recombinant hIL20.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant IL20 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 21.12 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3.
Expressed at very low levels in skin, trachea, and other tissues.