Anti-IL-2 Receptor alpha抗体[IL2R.1] (ab9496)
Key features and details
- Mouse monoclonal [IL2R.1] to IL-2 Receptor alpha
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-IL-2 Receptor alpha抗体[IL2R.1]
参阅全部 IL-2 Receptor alpha 一抗 -
描述
小鼠单克隆抗体[IL2R.1] to IL-2 Receptor alpha -
宿主
Mouse -
特异性
This antibody is specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages. -
经测试应用
适用于: Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-2 Receptor alpha.
Database link: P01589 -
阳性对照
- Tonsil.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.1% Sodium azide
Constituent: 0.1% Tween -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
IL2R.1 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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Flow Cyt |
1/50.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (1) |
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt
1/50. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/25 - 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Receptor for interleukin-2. -
疾病相关
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
序列相似性
Contains 2 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3559 Human
- Omim: 147730 Human
- SwissProt: P01589 Human
- Unigene: 231367 Human
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别名
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
图片
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Formalin-fixed, paraffin-embeded human tonsil tissue stained for IL-2 Receptor alpha using ab9496 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
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ab9496 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with CD25, using ABC and AEC chromagen.
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Overlay histogram showing Jurkat cells stained with ab9496 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9496, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab9496 被引用在 2 文献中.
- King JY et al. Pathway analysis of coronary atherosclerosis. Physiol Genomics 23:103-18 (2005). IHC-Fr ; Human . PubMed: 15942018
- Boyd JH et al. Upper airway muscle inflammation and denervation changes in obstructive sleep apnea. Am J Respir Crit Care Med 170:541-6 (2004). PubMed: 15151922