概述

  • 产品名称
    Anti-IL2抗体[EPR2780]
    参阅全部 IL2 一抗
  • 描述
    兔单克隆抗体[EPR2780] to IL2
  • 经测试应用
    适用于: WB, IHC-P, ICC, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human IL2.

  • 阳性对照
    • Human thymus and PBMC cell lysates, Human tonsil tissue; Jurkat cell lydate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-IL2 antibody (Alexa Fluor® 647) [EPR2780] (ab199215)

    Anti-CD99 antibody (Alexa Fluor® 488) [EPR3096] (ab208372)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR2780
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab92381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC 1/500 - 1/1000.
Flow Cyt 1/100.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • 疾病相关
      Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • 序列相似性
      Belongs to the IL-2 family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    图片

    • All lanes : Anti-IL2 antibody [EPR2780] (ab92381) at 1/1000 dilution

      Lane 1 : Human thymus lysate
      Lane 2 : PBMC lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
    • Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling IL2 with purified ab92381 at 1/100 dilution (10ug/mL) (red). Cells were fixed with 80% methanol and permeabilised using 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • ab92381 at 1/250 dilution staining IL2 in Formalin-fixed, Paraffin-embedded Human tonsil tissue. Detection: DAB staining.

    文献

    This product has been referenced in:
    • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »
    • Verma C  et al. Natural killer (NK) cell profiles in blood and tumour in women with large and locally advanced breast cancer (LLABC) and their contribution to a pathological complete response (PCR) in the tumour following neoadjuvant chemotherapy (NAC): differential restoration of blood profiles by NAC and surgery. J Transl Med 13:180 (2015). IHC . Read more (PubMed: 26040463) »

    See all 2 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab92381.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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