The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (2.0 ng/ml), a concentration of 0.04 - 0.05 µg/ml of this antibody is required.
ELISA:To detect mIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-2.
Western Blot:To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight in Human: 15 kDa.
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.