概述

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. To detect hIL-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-2.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hIL-2 (2.0 ng/ml), a concentration of 0.12 - 0.16 µg/ml of this antibody is required.

靶标

  • 功能
    Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
  • 疾病相关
    Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
  • 序列相似性
    Belongs to the IL-2 family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Aldesleukin antibody
    • IL 2 antibody
    • IL-2 antibody
    • IL2 antibody
    • IL2_HUMAN antibody
    • Interleukin 2 antibody
    • Interleukin-2 antibody
    • Involved in regulation of T cell clonal expansion antibody
    • Lymphokine antibody
    • OTTHUMP00000164090 antibody
    • POIL2 antibody
    • T Cell Growth Factor antibody
    • T-cell growth factor antibody
    • TCGF antibody
    see all

图片

  • All lanes : Anti-IL2 antibody (ab9618) at 1/1000 dilution

    Lane 1 : Recombinant human IL2 protein (ab73598) at 0.1 µg
    Lane 2 : Recombinant human IL2 protein (ab73598) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

实验方案

文献

This product has been referenced in:
  • LaMonica BE  et al. Mitotic spindle orientation predicts outer radial glial cell generation in human neocortex. Nat Commun 4:1665 (2013). Read more (PubMed: 23575669) »

See 1 Publication for this product

客户评价及客户问答

Thank you for your enquiry. Ab9618 has only been tested in a direct ELISA, as follows: To detect hIL-2 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity pur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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