概述

  • 产品名称Anti-IL2抗体
    参阅全部 IL2 一抗
  • 描述
    山羊多克隆抗体to IL2
  • 特异性The product shows no cross-reactivity with other cytokines tested using indirect ELISA.
  • 经测试应用适用于: ELISA, WB, Neutralisingmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant human Interleukin 2 (IL2), expressed in E. coli.

  • 阳性对照
  • 常规说明Endotoxin: <10 ng/vial by LAL method.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The product is purified by Protein G affinity chromatography.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
Neutralising
  • 应用说明ELISA: Use at a concentration of 1 µg/ml to detect 0.6 ng/well of recombinant human IL2.
    Neut: Use at a concentration of 0.8 - 8.0 µg/ml.
    WB: Use at a concentration of 2 µg/ml, this detects 5 ng/lane of recombinant human IL2 under reducing conditions and 20 ng/lane under non-reducing conditions.Predicted molecular weight: 19 kDa.

    Anti-Human IL2 is tested for its ability to neutralize the biological activity of recombinant, human IL2 on CTLL2 cells. The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, human IL2, when IL2 is present at a concentration just high enough to elicit a maximum response.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
    • 疾病相关Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.
    • 序列相似性Belongs to the IL-2 family.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Aldesleukin antibody
      • IL 2 antibody
      • IL-2 antibody
      • IL2 antibody
      • IL2_HUMAN antibody
      • Interleukin 2 antibody
      • Interleukin-2 antibody
      • Involved in regulation of T cell clonal expansion antibody
      • Lymphokine antibody
      • OTTHUMP00000164090 antibody
      • POIL2 antibody
      • T Cell Growth Factor antibody
      • T-cell growth factor antibody
      • TCGF antibody
      see all

    Anti-IL2 antibody (ab10752)参考文献

    This product has been referenced in:
    • Klein M  et al. Repression of cyclic adenosine monophosphate upregulation disarms and expands human regulatory T cells. J Immunol 188:1091-7 (2012). Neutralising ; Human . Read more (PubMed: 22190184) »

    See 1 Publication for this product

    Product Wall

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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    Thank you for forwarding some details of your protocol following our conversation this morning. I understand from the questionnaire you kindly sent that you have only attempted a sandwich ELISA experiment with ab10752. This application has not been tes...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"