概述

  • 产品名称Anti-IL1RAPL1抗体
    参阅全部 IL1RAPL1 一抗
  • 描述
    兔多克隆抗体to IL1RAPL1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Chicken, Human, Chimpanzee, Macaque Monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human IL1RAPL1 aa 650 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q9NZN1

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brain and Mouse Hippocampus tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 95 kDa (predicted molecular weight: 80 kDa).

靶标

  • 功能May regulate secretion and presynaptic differentiation through inhibition of the activity of N-type voltage-gated calcium channel. May activate the MAP kinase JNK.
  • 组织特异性Detected at low levels in heart, skeletal muscle, ovary, skin, amygdala, caudate nucleus, corpus callosum, hippocampus, substantia nigra and thalamus. Detected at very low levels in tonsil, prostate, testis, small intestine, placenta, colon and fetal liver.
  • 疾病相关Defects in IL1RAPL1 are the cause of mental retardation X-linked type 21 (MRX21) [MIM:300143]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
  • 序列相似性Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 细胞定位Cell membrane. Cytoplasm. May localize to the cell body and growth cones of dendrite-like processes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 1 RAPL 1 antibody
    • IL 1RAPL 1 antibody
    • IL-1-RAPL-1 antibody
    • IL-1RAPL-1 antibody
    • IL1R8 antibody
    • IL1RAPL 1 antibody
    • IL1RAPL antibody
    • IL1RAPL-1 antibody
    • IL1RAPL1 antibody
    • Interleukin 1 receptor 8 antibody
    • Interleukin 1 receptor accessory protein like 1 antibody
    • Interleukin-1 receptor accessory protein-like 1 antibody
    • IRPL1_HUMAN antibody
    • Mental retardation X linked 10 antibody
    • Mental retardation X linked 21 antibody
    • Mental retardation X linked 34 antibody
    • MRX 21 antibody
    • MRX 34 antibody
    • MRX10 antibody
    • MRX21 antibody
    • MRX34 antibody
    • Oligophrenin 4 antibody
    • Oligophrenin-4 antibody
    • Oligophrenin4 antibody
    • OPHN 4 antibody
    • OPHN4 antibody
    • Three immunoglobulin domain containing IL 1 receptor related 2 antibody
    • Three immunoglobulin domain-containing IL-1 receptor-related 2 antibody
    • TIGIRR 2 antibody
    • TIGIRR-2 antibody
    • TIGIRR2 antibody
    • X linked interleukin 1 receptor accessory protein like 1 antibody
    • X-linked interleukin-1 receptor accessory protein-like 1 antibody
    see all

Anti-IL1RAPL1 antibody 图像

  • All lanes : Anti-IL1RAPL1 antibody (ab117480) at 1/250 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Mouse Hippocampus Tissue Lysate
    Lane 3 : Brain (Mouse) Tissue Lysate with Immunising peptide at 1/250 dilution
    Lane 4 : Mouse Hippocampus Tissue Lysate with Immunising peptide at 1/250 dilution

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 103 kDa (possible non-specific binding),32 kDa (possible non-specific binding).

    Exposure time : 12 minutes

    IL1RAPL1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

     

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117480 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL1RAPL1 antibody (ab117480)参考文献

ab117480 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab117480.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"