概述

  • 产品名称Anti-IL1RA抗体[EPR6483]
    参阅全部 IL1RA 一抗
  • 描述
    兔单克隆抗体[EPR6483] to IL1RA
  • 经测试应用适用于: WB, IP, IHC-P, Flow Cyt, ICC/IF, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IL1RA aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: A431, 293T, MOLT4, HeLa, RAW264.7, NIH/3T3, and L6 cell lysates. IHC-P: Human, mouse and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: A431 and HeLa cells. IP: NIH/3T3 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-IL1RA antibody (Biotin) [EPR6483] (ab205855)

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 解离常数(KD KD = 8.40 x 10 -12 M Learn more about KD
  • 存储溶液pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号EPR6483
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab124962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
  • 组织特异性The intracellular form of IL1RN is predominantly expressed in epithelial cells.
  • 疾病相关Microvascular complications of diabetes 4
    Interleukin 1 receptor antagonist deficiency
  • 细胞定位Cytoplasm and Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DIRA antibody
    • ICIL 1RA antibody
    • ICIL-1RA antibody
    • ICIL1RA antibody
    • IL-1ra antibody
    • IL-1ra3 antibody
    • IL-1RN antibody
    • IL1 inhibitor antibody
    • IL1F3 antibody
    • IL1RA antibody
    • IL1RA_HUMAN antibody
    • IL1RN (IL1F3) antibody
    • IL1RN antibody
    • Interleukin 1 receptor antagonist antibody
    • Interleukin-1 receptor antagonist protein antibody
    • Intracellular IL 1 receptor antagonist type II antibody
    • Intracellular interleukin 1 receptor antagonist (icIL 1ra) antibody
    • IRAP antibody
    • MGC10430 antibody
    • MVCD4 antibody
    • Type II interleukin 1 receptor antagonist antibody
    see all

Anti-IL1RA antibody [EPR6483] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IL1RA with unpurified ab124962 at a dilution of 1/100.

  • Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124962 at a dilution of 1 in 20 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling IL1RA with purified ab124962 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

  • Anti-IL1RA antibody [EPR6483] (ab124962) at 20 µg (purified) + L6 whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

    Blocking and dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-IL1RA antibody [EPR6483] (ab124962) at 20 µg (purified)

    Lane 1 : A431 whole cell lysate
    Lane 2 : HEK293 whole cell lysate
    Lane 3 : Molt-4 whole cell lysate
    Lane 4 : HeLa whole cell lysate
    Lane 5 : Raw264.7 whole cell lysate
    Lane 6 : NIH/3T3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 20 kDa
    Observed band size : 20 kDa

    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling IL1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IL1RA with purified ab124962 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry analysis of HeLa cells labelling IL1RA with purified ab124962 at 1/20 (red). Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • ab124962 (purified) at 1/20 immunoprecipitating IL1RA in NIH/3T3 whole cell lysate.

    Lane 1 (input): NIH/3T3 whole cell lysate (10µg)

    Lane 2 (+): ab124962 + NIH/3T3 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124962 in NIH/3T3 whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-IL1RA antibody [EPR6483] (ab124962) at 1/10000 dilution (unpurified)

    Lane 1 : A431 cell lysates
    Lane 2 : 293T cell lysates
    Lane 3 : MOLT4 cell lysates
    Lane 4 : HeLa cell lysates
    Lane 5 : RAW264.7 cell lysates
    Lane 6 : NIH 3T3 cell lysates
    Lane 7 : L6 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size : 20 kDa
  • Overlay histogram showing A431 cells stained with unpurified ab124962 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab124962, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-IL1RA antibody [EPR6483] (ab124962)参考文献

This product has been referenced in:
  • Oh YS  et al. Effects of bilateral vasectomy on the interleukin 1 system in mouse epididymis. Am J Reprod Immunol N/A:N/A (2016). IHC . Read more (PubMed: 27476761) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Biocare Rodent Block R as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Sample Rat Tissue sections (rat ligament)
Specification rat ligament
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

提交于 May 28 2014

Application ELISA
Sample Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
Specification St. protein from Peprotech ELISA development kit ,
Type Sandwich (Capture)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Toomas Neuman

Verified customer

提交于 Jan 29 2014

Application ELISA
Sample Human Recombinant protein (St. protein from Peprotech ELISA development kit ,)
Specification St. protein from Peprotech ELISA development kit ,
Username

Mr. Toomas Neuman

Verified customer

提交于 Jan 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"