Recombinant protein fragment containing a sequence corresponding to a region within amino acids 45 and 177 of IL1RA (NP_776214)
293T, A431, HeLaS3, HepG2 and Raji cell lysate. Molt-4 whole cell lysate. HeLa cells.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
1/100 - 1/200.
Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
Microvascular complications of diabetes 4
Interleukin 1 receptor antagonist deficiency
Cytoplasm and Secreted.
Information by UniProt
ICIL 1RA antibody
Western blot - Anti-IL1RA antibody (ab97301)
Anti-IL1RA antibody (ab97301) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size: 20 kDa 12% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-IL1RA antibody (ab97301)
Immunofluorescence analysis of methanol-fixed HeLa, using ab97301 at 1/200 dilution. Lower image costained with Hoechst 33342.
has not yet been referenced specifically in any publications.
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