概述

  • 产品名称Anti-IL18R1抗体
    参阅全部 IL18R1 一抗
  • 描述
    兔多克隆抗体to IL18R1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human IL18R1 aa 500 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q13478

  • 阳性对照
    • This antibody gave a positive signal in both Human Spleen and Lung tissue lysates as well as Hodgkin's Lymphoma (Human) Whole Cell Lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab117432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 62 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Receptor for interleukin 18 (IL-18). Binding to the agonist leads to the activation of NF-kappa-B.
  • 组织特异性Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.
  • 序列相似性Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD218 antigen-like family member A antibody
    • CD218a antibody
    • CDw218a antibody
    • CDw218a antigen antibody
    • IL 1Rrp antibody
    • IL-18R-1 antibody
    • IL-18R1 antibody
    • IL-1Rrp antibody
    • IL1 receptor related protein antibody
    • IL1 receptor-related protein antibody
    • IL18R_HUMAN antibody
    • IL18R1 antibody
    • IL18RA antibody
    • Il18ralpha antibody
    • IL1R-rp antibody
    • IL1RRP antibody
    • Interleukin 18 receptor 1 antibody
    • Interleukin 18 receptor, alpha chain antibody
    • Interleukin-18 receptor 1 antibody
    see all

Anti-IL18R1 antibody 图像

  • IHC image of IL18R1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117432, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-IL18R1 antibody (ab117432) at 1 µg/ml + Hodgkin's Lymphoma (Human) Whole Cell Lysate - tumor tissue at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    The band observed at 60 kDa could potentially be a cleaved form of IL18R1 due to the presence of an 18 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117432 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • All lanes : Anti-IL18R1 antibody (ab117432) at 1 µg/ml

    Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    The band observed at 60 kDa could potentially be a cleaved form of IL18R1 due to the presence of an 18 amino acid signal peptide.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab117432 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-IL18R1 antibody (ab117432)参考文献

ab117432 has not yet been referenced specifically in any publications.

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