概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL17RE
  • 经测试应用
    适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human IL17RE.

    (Peptide available as ab93797.)

  • 阳性对照
    • This antibody gave a positive signal in Jurkat whole cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 71 kDa (predicted molecular weight: 74 kDa).

靶标

  • 相关性
    Receptor which can activate the MAPK signaling pathway and seems to function at, or upstream of Ras. Isoform 2 and isoform 4 may be either cytoplasmic inactive or dominant active forms. Isoform 3 and isoform 5 may act as soluble decoy receptors
  • 细胞定位
    Membrane (isoform 1), Cytoplasm (isoforms 2 + 4), Secreted (isoforms 3 + 5)
  • 数据库链接
  • 别名
    • FLJ23658 antibody
    • IL-17 receptor E antibody
    • IL-17RE antibody
    • interleukin 17 receptor E antibody
    • Interleukin-17 receptor E antibody
    • MGC71884 antibody
    • OTTHUMP00000158822 antibody
    • UNQ3056/PRO9877 antibody
    see all

图片

  • Anti-IL17RE antibody (ab77527) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 71 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • IHC image of IL17RE staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77527, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

文献

This product has been referenced in:
  • Tenland E  et al. Innate Immune Responses after Airway Epithelial Stimulation with Mycobacterium bovis Bacille-Calmette Guérin. PLoS One 11:e0164431 (2016). Flow Cyt ; Human . Read more (PubMed: 27723804) »

See 1 Publication for this product

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