概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL17D
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17D.

    (Peptide available as ab90991.)

  • 阳性对照
    • This antibody gave a positive signal in the following lysates: Human Thymus Tissue; Jurkat Whole Cell

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能
    Induces expression of IL-6, IL-8, and GM-CSF from endothelial cells.
  • 组织特异性
    Expressed preferentially in adipose, skeletal muscle and CNS.
  • 序列相似性
    Belongs to the IL-17 family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 17D antibody
    • IL 22 antibody
    • IL 27 antibody
    • IL-17D antibody
    • IL-27 antibody
    • IL17D antibody
    • IL17D_HUMAN antibody
    • IL27 antibody
    • Interleukin 17D antibody
    • Interleukin 27 antibody
    • Interleukin-17D antibody
    • Interleukin-27 antibody
    see all

Anti-IL17D antibody 图像

  • All lanes : Anti-IL17D antibody (ab77185) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of 77185 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77185, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-IL17D antibody (ab77185)参考文献

This product has been referenced in:
  • Bhowmick D  et al. Para-Phenylenediamine Induces Apoptotic Death of Melanoma Cells and Reduces Melanoma Tumour Growth in Mice. Biochem Res Int 2016:3137010 (2016). Read more (PubMed: 27293892) »

See 1 Publication for this product

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