概述

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-17 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.1 - 0.18 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-17 (6.0 ng/ml).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • 组织特异性
    Restricted to activated memory T-cells.
  • 序列相似性
    Belongs to the IL-17 family.
  • 翻译后修饰
    Found both in glycosylated and nonglycosylated forms.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

文献

This product has been referenced in:
  • Liu L  et al. Periductal Mastitis: An Inflammatory Disease Related to Bacterial Infection and Consequent Immune Responses? Mediators Inflamm 2017:5309081 (2017). Read more (PubMed: 28182101) »
  • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »

See all 7 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Small bowel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1 (EDTA pH8.5)
Permeabilization
No
Specification
Small bowel
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 06 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Goat Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6.0)
Sample
Human Tissue sections (Colonic Mucosa)
Specification
Colonic Mucosa
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 02 2013

Thank you for your enquiry regarding ab9565.

We do not have any recommendations for lysis buffers, as we do not test this antibody on cell lysate in our labs. As the datasheet indicates, this antibody has been tested on recombinant hIL-17 at ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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