概述

性能

  • 形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型unknown
  • 轻链类型unknown
  • 研究领域

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应用

Our Abpromise guarantee covers the use of ab9565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-17 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.1 - 0.18 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-17 (6.0 ng/ml).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • 组织特异性Restricted to activated memory T-cells.
  • 序列相似性Belongs to the IL-17 family.
  • 翻译后修饰Found both in glycosylated and nonglycosylated forms.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Anti-IL17 antibody (ab9565)参考文献

This product has been referenced in:
  • Kaewkangsadan V  et al. Crucial Contributions by T Lymphocytes (Effector, Regulatory, and Checkpoint Inhibitor) and Cytokines (TH1, TH2, and TH17) to a Pathological Complete Response Induced by Neoadjuvant Chemotherapy in Women with Breast Cancer. J Immunol Res 2016:4757405 (2016). IHC-P ; Human . Read more (PubMed: 27777963) »
  • Harvey BS  et al. Interleukin 17A evoked mucosal damage is attenuated by cannabidiol and anandamide in a human colonic explant model. Cytokine 65:236-44 (2014). WB, IHC-P ; Human . Read more (PubMed: 24238999) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Goat Serum as blocking agent for 30 minute(s) · Concentration: 50% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6.0)
Sample Human Tissue sections (Colonic Mucosa)
Specification Colonic Mucosa
Permeabilization Yes - 0.1% Triton X-100
Fixative Formaldehyde
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Abcam user community

Verified customer

提交于 Jul 02 2013

Thank you for your enquiry regarding ab9565.

We do not have any recommendations for lysis buffers, as we do not test this antibody on cell lysate in our labs. As the datasheet indicates, this antibody has been tested on recombinant hIL-17 at ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"