概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL17
  • 经测试应用
    适用于: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human IL17 (internal sequence). ab79056 was raised against a 19 amino acid synthetic peptide from near the center of human IL-17A.
    Database link: Q16552
    (Peptide available as ab190733)

  • 阳性对照
  • 常规说明

    The blocking peptide is available as ab190773

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium azide
    Constituent: 99.98% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
  • 组织特异性
    Restricted to activated memory T-cells.
  • 序列相似性
    Belongs to the IL-17 family.
  • 翻译后修饰
    Found both in glycosylated and nonglycosylated forms.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CTLA 8 antibody
    • CTLA-8 antibody
    • CTLA8 antibody
    • Cytotoxic T lymphocyte associated antigen 8 antibody
    • Cytotoxic T lymphocyte associated protein 8 antibody
    • Cytotoxic T lymphocyte associated serine esterase 8 antibody
    • Cytotoxic T-lymphocyte-associated antigen 8 antibody
    • IL 17A antibody
    • IL-17 antibody
    • IL-17A antibody
    • IL17 antibody
    • IL17_HUMAN antibody
    • Il17a antibody
    • Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8) antibody
    • Interleukin 17A antibody
    • Interleukin-17A antibody
    • Interleukin17 antibody
    • Interleukin17A antibody
    • OTTHUMP00000016597 antibody
    • OTTMUSP00000046003 antibody
    see all

Anti-IL17 antibody 图像

  • Paraffin-embedded human tonsil tissue stained for IL17 using ab79056 at 5 µg/ml in immunohistochemical analysis.

  • Anti-IL17 antibody (ab79056) at 1 µg/ml + Recombinant human IL-17 at 0.001 µg

    Predicted band size : 18 kDa
  • ab79056 staining IL17 in Dog lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with Tween-20 (0.05%) and blocked with 10% serum for 2 hours at 24°C; antigen retrieval was by heat mediation with citrate buffer (0.01M). Samples were incubated with primary antibody (1/100 in PBS +10% serum) for 14 hours at 4°C. A Biotin-conjugated Horse pan-specific anti-IgG polyclonal (1/20) was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-IL17 antibody (ab79056) at 0.5 µg/ml
    Lane 2 : Anti-IL17 antibody (ab79056) at 1 µg/ml

    Lane 1 : A-20 cell lysate
    Lane 2 : A-20 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Additional bands at : 22 kDa,28 kDa,32 kDa. We are unsure as to the identity of these extra bands.The higher molecular weight bands seen in the Western blot may represent glycosylated IL17.
  • All lanes : Anti-IL17 antibody (ab79056)

    Lane 1 : human thymus tissue lysate at 1 µg/ml
    Lane 2 : human thymus tissue lysate at 2 µg/ml


    Predicted band size : 18 kDa

Anti-IL17 antibody (ab79056)参考文献

This product has been referenced in:
  • Liu X  et al. Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model. Int J Chron Obstruct Pulmon Dis 12:1309-1324 (2017). WB ; Rat . Read more (PubMed: 28496315) »
  • Zhang H  et al. Toll-Like Receptor 4-Myeloid Differentiation Primary Response Gene 88 Pathway Is Involved in the Inflammatory Development of Polymyositis by Mediating Interferon-? and Interleukin-17A in Humans and Experimental Autoimmune Myositis Mouse Model. Front Neurol 8:132 (2017). IHC . Read more (PubMed: 28446897) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (stomach)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formain
Username

Abcam user community

Verified customer

提交于 May 08 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
10% normal buffered formalin
Username

Abcam user community

Verified customer

提交于 May 08 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 25% · Temperature: 37°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat
Sample
Rhesus monkey Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
Glutaraldehyde
Username

Ms. Maria Daskalaki

Verified customer

提交于 Nov 13 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: microwave with citrate buffer pH 6.0
Sample
Cow Tissue sections (Mammary gland)
Specification
Mammary gland
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Natalia Salvetti

Verified customer

提交于 Oct 09 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (salivary gland)
Specification
salivary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Tris pH=9.0 / 1mM EDTA
Permeabilization
No
Username

Abcam user community

Verified customer

提交于 Jan 09 2013

Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications and species listed on the datasheets. Therefore, any IL17 antibody teste...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver whole extract)
Loading amount
100 µg
Specification
Liver whole extract
Gel Running Conditions
Reduced Denaturing (14% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

提交于 Sep 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Colon)
Specification
Colon
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Diva Decloaker
Permeabilization
No
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (tonsil)
Specification
tonsil
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 21 2012

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: DAKO antigen retrieval buffer , pressure cooked for 24 minutes
Permeabilization
Yes - 0.3% Triton X-100, 5 min RT
Blocking step
(agent) for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Minsha Manoharan

Verified customer

提交于 Aug 15 2012

1-10 of 32 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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