产品名称 Anti-IL17抗体 参阅全部 IL17 一抗
WB more details
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human IL17.
(Peptide available as
This antibody gave a positive signal in the following lysates: Thymus Tissue Lysate; U937 Whole Cell Lysate; THP1 Whole Cell Lysate; Raji Whole Cell Lysate; Ramos Whole Cell Lysate; Lymph node Tissue Lysate; Tonsil Tissue Lysate.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
功能 Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines (PubMed:8676080).
组织特异性 Restricted to activated memory T-cells.
序列相似性 Belongs to the IL-17 family.
翻译后修饰 Found both in glycosylated and nonglycosylated forms.
Information by UniProt
CTLA 8 antibody
Anti-IL17 antibody 图像
Western blot - IL17 antibody (ab77171)
All lanes : Anti-IL17 antibody (ab77171) at 1 µg/ml Lane 1 : Human thymus tissue lysate - total protein ( ab30146) Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate Lane 4 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 5 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 6 : Human lymph node tissue lysate - total protein ( ab29871) Lane 7 : Tonsil (Human) Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution developed using the ECL technique Performed under reducing conditions. Predicted band size : 18 kDa Observed band size : 18 kDa Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands. Exposure time : 30 seconds
Western blot - IL17 protein (Active) ( ab73202)
Anti-IL17 antibody (ab77171) at 1 µg/ml + Recombinant human IL17 protein ( ab73202) at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ( ab97080) at 1/5000 dilution developed using the ECL technique Performed under reducing conditions. Exposure time : 1 minute
Anti-IL17 antibody (ab77171)参考文献
has not yet been referenced specifically in any publications.
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