概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL16
  • 经测试应用
    适用于: ELISA, Neutralising, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hIL-16 (human Interleukin-16)

性能

  • 形式
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • 存储溶液
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    unknown
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab9563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
Neutralising
WB
  • 应用说明
    Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2 ng/ml), a concentration of 0.07 - 0.012 µg/ml of this antibody is required.

    ELISA:To detect hIL-16 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-16.

    Western Blot:To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
  • 靶标

    • 功能
      Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
      Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
      Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.
    • 组织特异性
      Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation.
    • 序列相似性
      Contains 4 PDZ (DHR) domains.
    • 翻译后修饰
      Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.
    • 细胞定位
      Cytoplasm; Cytoplasm. Nucleus and Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HGNC:5980 antibody
      • HsT19289 antibody
      • IL-16 antibody
      • IL16 antibody
      • IL16_HUMAN antibody
      • Interleukin 16 precursor antibody
      • Interleukin-16 antibody
      • LCF antibody
      • Lymphocyte chemoattractant factor antibody
      • Neuronal interleukin 16 antibody
      • NIL16 antibody
      • prIL 16 antibody
      • PrIL16 antibody
      • Prointerleukin 16 antibody
      see all

    实验方案

    Anti-IL16 antibody (ab9563)参考文献

    This product has been referenced in:
    • Ahn DS  et al. Secretion of IL-16 through TNFR1 and calpain-caspase signaling contributes to MRSA pneumonia. Mucosal Immunol N/A:N/A (2014). Read more (PubMed: 24736233) »

    See 1 Publication for this product

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Sample
    Mouse Cell (Hepatocyte)
    Specification
    Hepatocyte
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Mar 21 2014

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Non-reduced Denaturing (15%)
    Sample
    Mouse Cell lysate - whole cell (primary hepatoctyes)
    Specification
    primary hepatoctyes
    Blocking step
    I-Block(Applied biosystems) as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 09 2013


    The immunogen sequence of ab9563 is:
    MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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