概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL15
  • 经测试应用
    适用于: Blocking, ICC/IF, Neutralising, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant full length protein (Mouse). Expressed in E. coli

  • 常规说明


    IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • Primary antibody说明
    IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking Use a concentration of 10 µg/ml. PubMed: 17239632
ICC/IF Use a concentration of 1 µg/ml. PubMed: 15921960
Neutralising Use at an assay dependent concentration.
WB 1/500.

靶标

  • 功能
    Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
  • 组织特异性
    Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
  • 序列相似性
    Belongs to the IL-15/IL-21 family.
  • 细胞定位
    Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 15 antibody
    • IL-15 antibody
    • IL15 antibody
    • IL15_HUMAN antibody
    • Interleukin 15 antibody
    • Interleukin-15 antibody
    • Interleukin15 antibody
    • MGC9721 antibody
    see all

文献

This product has been referenced in:
  • de la Rica L  et al. NF-?B-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol 16:2 (2015). WB . Read more (PubMed: 25601191) »
  • Winau F  et al. Ito cells are liver-resident antigen-presenting cells for activating T cell responses. Immunity 26:117-29 (2007). Blocking ; Human, Mouse . Read more (PubMed: 17239632) »

See all 3 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small intestine)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: alpha chemotrypsin
Specification
Small intestine
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
10% formalin
Username

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Verified customer

提交于 Jun 02 2017

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab7213 in Flow Cytometry on Mouse. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submi...

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Thank you for your enquiry. All the information that we have regarding ab7213 is located on the online datasheet. I'm sorry, but it does not appear that we have information about a positive control for this particular antibody. If you have any more que...

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Sorry, we do not have a biotinylated form of this antibody, and we do not have a service to perfom this.

This antibody has been shown to detect mouse IL-15 in WB. The only references we are aware of are those listed on the datasheet.

The immunogen used to create this antibody was Mouse IL-15. The antibody is a polyclonal and will therefore recognise a number of epitopes on the surface of the immunogen. As far as we are aware, the antibody has not been tested in flow cytometry.

This antibody is neutralizing, but you will have to establish your own conditions.

This antibody has not been tested for neutralization.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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