概述

  • 产品名称Anti-IL15抗体
    参阅全部 IL15 一抗
  • 描述
    小鼠单克隆抗体to IL15
  • 经测试应用适用于: IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab55276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.
  • 组织特异性Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.
  • 序列相似性Belongs to the IL-15/IL-21 family.
  • 细胞定位Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.
  • Information by UniProt
  • 数据库链接
  • 别名
    • IL 15 antibody
    • IL-15 antibody
    • IL15 antibody
    • IL15_HUMAN antibody
    • Interleukin 15 antibody
    • Interleukin-15 antibody
    • Interleukin15 antibody
    • MGC9721 antibody
    see all

Anti-IL15 antibody 图像

  • IL15 antibody (ab55276) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer.
  • Overlay histogram showing JEG3 cells stained with ab55276 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55276, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-IL15 antibody (ab55276)参考文献

This product has been referenced in:
  • Herndler-Brandstetter D  et al. Human bone marrow hosts polyfunctional memory CD4+ and CD8+ T cells with close contact to IL-15-producing cells. J Immunol 186:6965-71 (2011). IHC-P ; Human . Read more (PubMed: 21562158) »
  • Zhang Z  et al. Hypercytolytic activity of hepatic natural killer cells correlates with liver injury in chronic hepatitis B patients. Hepatology 53:73-85 (2011). IHC-P ; Human . Read more (PubMed: 21254163) »

See all 3 Publications for this product

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