Anti-IL-15抗体[3A3] (ab55276)
Key features and details
- Mouse monoclonal [3A3] to IL-15
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-IL-15抗体[3A3]
参阅全部 IL-15 一抗 -
描述
小鼠单克隆抗体[3A3] to IL-15 -
宿主
Mouse -
经测试应用
适用于: IHC-P, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human IL-15.
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常规说明
This product was changed from ascites to tissue culture supernatant on 11th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
3A3 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab55276于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha. -
组织特异性
Most abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus. -
序列相似性
Belongs to the IL-15/IL-21 family. -
细胞定位
Secreted and Cytoplasm. Nucleus. IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components. - Information by UniProt
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数据库链接
- Entrez Gene: 3600 Human
- Omim: 600554 Human
- SwissProt: P40933 Human
- Unigene: 654378 Human
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别名
- IL 15 antibody
- IL-15 antibody
- IL15 antibody
see all
图片
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IL-15 antibody (ab55276) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer.
This image was generated using the ascites version of the product.
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Overlay histogram showing JEG3 cells stained with ab55276 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55276, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (8)
ab55276 被引用在 8 文献中.
- Nakamizo S et al. Single-cell analysis of human skin identifies CD14+ type 3 dendritic cells co-producing IL1B and IL23A in psoriasis. J Exp Med 218:N/A (2021). PubMed: 34279540
- Kinjyo I et al. Leukemia-derived exosomes and cytokines pave the way for entry into the brain. J Leukoc Biol 105:741-753 (2019). PubMed: 30702754
- Chen X et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
- Pangrazzi L et al. Increased IL-15 Production and Accumulation of Highly Differentiated CD8(+) Effector/Memory T Cells in the Bone Marrow of Persons with Cytomegalovirus. Front Immunol 8:715 (2017). PubMed: 28674537
- Chen Y et al. Human fused NKG2D-IL-15 protein controls xenografted human gastric cancer through the recruitment and activation of NK cells. Cell Mol Immunol 14:293-307 (2017). PubMed: 26364916
- Gulic T et al. Potential role of heat-shock protein 70 and interleukin-15 in the pathogenesis of threatened spontaneous abortions. Am J Reprod Immunol 76:126-36 (2016). PubMed: 27225940
- Herndler-Brandstetter D et al. Human bone marrow hosts polyfunctional memory CD4+ and CD8+ T cells with close contact to IL-15-producing cells. J Immunol 186:6965-71 (2011). IHC-P ; Human . PubMed: 21562158
- Zhang Z et al. Hypercytolytic activity of hepatic natural killer cells correlates with liver injury in chronic hepatitis B patients. Hepatology 53:73-85 (2011). IHC-P ; Human . PubMed: 21254163