Anti-IL13 receptor alpha 2抗体(ab55275)

概述

性能

应用

Our Abpromise guarantee covers the use of ab55275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 44 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.
IP 1/200.
ICC/IF Use a concentration of 1 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.

靶标

  • 功能
    Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
  • 序列相似性
    Belongs to the type I cytokine receptor family. Type 5 subfamily.
    Contains 1 fibronectin type-III domain.
  • 结构域
    The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cancer/testis antigen 19 antibody
    • CD213a2 antibody
    • CT19 antibody
    • I13R2_HUMAN antibody
    • IL-13 receptor subunit alpha-2 antibody
    • IL-13R subunit alpha-2 antibody
    • IL-13R-alpha-2 antibody
    • IL-13RA2 antibody
    • IL13 receptor antibody
    • IL13BP antibody
    • IL13R antibody
    • Il13ra2 antibody
    • Interleukin 13 binding protein antibody
    • Interleukin 13 receptor alpha 2 chain antibody
    • Interleukin-13 receptor subunit alpha-2 antibody
    • Interleukin-13-binding protein antibody
    see all

图片

  • ab55275 staining IL13 rexeptor alpha 2 in Rabbit ear wound exudate tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 100% Protein block (serum free) for 30 minutes at 37°C; antigen retrieval was enzymatic. Samples were incubated with primary antibody (1µg/ml) for 1 hour at 37°C. An undiluted HRP-conjugated Mouse anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IL13 receptor alpha 2 antibody (ab55275) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 44 kDa
    IL13 receptor alpha 2 antibody (ab55275) at 1ug/ml + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab55275 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55275, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.

文献

This product has been referenced in:
  • Pollack IF  et al. Antigen-specific immunoreactivity and clinical outcome following vaccination with glioma-associated antigen peptides in children with recurrent high-grade gliomas: results of a pilot study. J Neurooncol 130:517-527 (2016). Read more (PubMed: 27624914) »
  • Witusik-Perkowska M  et al. Astrocytoma-associated antigens - IL13Ra2, Fra-1, and EphA2 as potential markers to monitor the status of tumour-derived cell cultures in vitro. Cancer Cell Int 14:82 (2014). Read more (PubMed: 25788865) »

See all 3 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Antigen retrieval step
Enzymatic
Sample
Rabbit Tissue sections (Ear wound exudates)
Specification
Ear wound exudates
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Harshini Sarojini

Verified customer

提交于 Oct 02 2013

Thank you for contacting us.
The molecular weight of Anti-IL13 receptor alpha 2 antibody (ab55275) is around 150KDa.
I hope this helps and if we can assist further, please do not hesitate to contact us.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Total protein in input
200 µg
Specification
AGS Gastric Carcinoma cells
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

提交于 Jun 03 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (AGS Gastric Carcinoma Cells)
Specification
AGS Gastric Carcinoma Cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (AGS Gastric Carcinoma cells)
Specification
AGS Gastric Carcinoma cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.025% Triton-X in TBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Apr 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Stomach)
Specification
Stomach
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (AGS)
Loading amount
10 µg
Specification
AGS
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Sep 23 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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