Anti-IL13 receptor alpha 1抗体[GM-1C8] (ab3772)

概述

  • 产品名称Anti-IL13 receptor alpha 1抗体[GM-1C8]
    参阅全部 IL13 receptor alpha 1 一抗
  • 描述
    小鼠单克隆抗体[GM-1C8] to IL13 receptor alpha 1
  • 特异性This antibody recognises IL-13 receptor alpha 1 transiently expressed on the cell surface of transfected BOSC cells as well as the native protein on monocytes (W. Luttmann, unpublished data). Specificity is routinely tested by flow cytometry on BOSC cells transiently transfected with an IL-13 receptor alpha 1 expression vector.
  • 经测试应用适用于: ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human IL-13 receptor alpha 1 chain extracellular domain cDNA (see relevance text).

  • 常规说明


    Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13 receptor alpha 1 together with IL-4 receptor alpha 1 forms a functional receptor for both IL-4and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液PBS pH 7.2, 0.01% sodium azide
  • Concentration information loading...
  • 纯度Protein G purified
  • Primary antibody说明Interleukin 13 (IL-13) is a T cell derived cytokine involved in the regulation of inflammatory and immune responses. IL-13 receptor alpha 1 together with IL-4 receptor alpha 1 forms a functional receptor for both IL-4and IL-13, which is why these two cytokines share many of their biological activities. The receptor is found on human B cells, monocytes and endothelial cells. However, no functional receptor is expressed on T cells, which explains why IL-13, in contrast to IL-4, fails to induce TH2-cell differentiation. Antibodies produced from cDNA: Conventional technologies usually either generate antibodies against purified proteins, or against synthetic peptides based on amino acid sequences derived from DNA sequence data. Genetic immunization involves introducing the gene in the form of a cDNA directly into an animal which translates this cDNA into protein thus stimulating an immune response against the foreign protein. Although the synthetic peptide approach is comparable in speed, the quality of antibodies generated by genetic immunization is far superior. This is because the protein is made by the immunized animal, utilzing complex cellular mechanisms that allow it to gain a native conformation. Antibodies are then generated against a native protein, such as is found in the blood or tissues of its host species. Membrane-bound or secreted proteins often create problems for conventional antibody technology because in their native form, they are often modified by glycosylation, or in some cases exist as multiple membrane-spanning proteins that are not soluble following isolation or synthesis in recombinant systems. All of these problems are avoided if the immunized animal makes the protein itself. Antibodies generated by genetic immunization have been shown to have binding affinities to the protein in the sub-nanomolar range, which are approximately 100x higher than conventionally developed antibodies and much higher than single chain antibodies. Results confirm published data for much higher avidity of sera generated by genetic immunization as compared with that gained by immunization with a corresponding recombinant protein.
  • 克隆单克隆
  • 克隆编号GM-1C8
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

靶标

  • 功能Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
  • 组织特异性Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
    • Cancer/testis antigen 19 antibody
    • CD213a1 antibody
    • CD213A1 Antigen antibody
    • CT 19 antibody
    • CT19 antibody
    • I13R1_HUMAN antibody
    • IL 13 receptor subunit alpha 1 antibody
    • IL 13R alpha 1 antibody
    • IL 13R subunit alpha 1 antibody
    • IL 13Ra antibody
    • IL 13RA1 antibody
    • IL-13 receptor subunit alpha-1 antibody
    • IL-13R subunit alpha-1 antibody
    • IL-13R-alpha-1 antibody
    • IL-13RA1 antibody
    • IL13 receptor alpha 1 chain antibody
    • IL13R antibody
    • IL13RA antibody
    • Il13ra1 antibody
    • Interleukin 13 receptor alpha 1 antibody
    • Interleukin 13 receptor alpha 1 chain antibody
    • interleukin 13 receptor subunit alpha 1 antibody
    • Interleukin-13 receptor subunit alpha-1 antibody
    • NR 4 antibody
    • NR4 antibody
    see all

Anti-IL13 receptor alpha 1 antibody [GM-1C8] (ab3772)参考文献

This product has been referenced in:
  • Kunii R  et al. Expression of CD13/aminopeptidase N on human gingival fibroblasts and up-regulation upon stimulation with interleukin-4 and interleukin-13. J Periodontal Res 40:138-46 (2005). Read more (PubMed: 15733148) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry and your interest in our product. The optimal dilutions / concentrations should be determined by the end user. As a general guideline, we would suggest testing this antibody at a concentration range of 0.1-10 ug/ml.

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with species ot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"