Anti-IL13 receptor alpha 1抗体(ab79277)

概述

  • 产品名称Anti-IL13 receptor alpha 1抗体
    参阅全部 IL13 receptor alpha 1 一抗
  • 描述
    兔多克隆抗体to IL13 receptor alpha 1
  • 经测试应用适用于: WB, IHC-P, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Mouse, Human, Rhesus monkey
  • 免疫原

    Synthetic non-phosphopeptide derived from human IL13 receptor alpha 1 around the phosphorylation site of tyrosine 405 (D-I-YP-E-K).

  • 阳性对照
    • NIH3T3 cells; COLO, HT29, A549 and 293 cell extracts; human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab79277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/40000.

靶标

  • 功能Binds with low affinity to interleukin-13 (IL13). Together with IL4RA can form a functional receptor for IL13. Also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.
  • 组织特异性Ubiquitous. Highest levels in heart, liver, skeletal muscle and ovary; lowest levels in brain, lung and kidney. Also found in B-cells, T-cells and endothelial cells.
  • 序列相似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bB128O4.2.1 (interleukin 13 receptor, alpha 1) antibody
    • Cancer/testis antigen 19 antibody
    • CD213a1 antibody
    • CD213A1 Antigen antibody
    • CT 19 antibody
    • CT19 antibody
    • I13R1_HUMAN antibody
    • IL 13 receptor subunit alpha 1 antibody
    • IL 13R alpha 1 antibody
    • IL 13R subunit alpha 1 antibody
    • IL 13Ra antibody
    • IL 13RA1 antibody
    • IL-13 receptor subunit alpha-1 antibody
    • IL-13R subunit alpha-1 antibody
    • IL-13R-alpha-1 antibody
    • IL-13RA1 antibody
    • IL13 receptor alpha 1 chain antibody
    • IL13R antibody
    • IL13RA antibody
    • Il13ra1 antibody
    • Interleukin 13 receptor alpha 1 antibody
    • Interleukin 13 receptor alpha 1 chain antibody
    • interleukin 13 receptor subunit alpha 1 antibody
    • Interleukin-13 receptor subunit alpha-1 antibody
    • NR 4 antibody
    • NR4 antibody
    see all

Anti-IL13 receptor alpha 1 antibody 图像

  • ab79277 at 1/50 dilution staining IL13 receptor alpha 1 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • ab79277 at 1/500 dilution staining IL13 receptor alpha 1 in NIH3T3 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-IL13 receptor alpha 1 antibody (ab79277) at 1/500 dilution

    Lane 1 : COLO cell extracts
    Lane 2 : HT29 cell extracts
    Lane 3 : A549 cell extracts
    Lane 4 : 293 cell extracts
    Lane 5 : COLO cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-IL13 receptor alpha 1 antibody (ab79277)参考文献

This product has been referenced in:
  • Goh YP  et al. Eosinophils secrete IL-4 to facilitate liver regeneration. Proc Natl Acad Sci U S A 110:9914-9 (2013). Read more (PubMed: 23716700) »
  • Barderas R  et al. High expression of IL-13 receptor a2 in colorectal cancer is associated with invasion, liver metastasis, and poor prognosis. Cancer Res 72:2780-90 (2012). Human . Read more (PubMed: 22505647) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (head and neck cancer)
Permeabilization Yes - triton x 100
Specification head and neck cancer
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 26°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rhesus monkey Cell (Colon)
Specification Colon
Fixative Formaldehyde
Blocking step 3% BSA and 10% Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 21 2013

Thank you for confirming these details and for your cooperation. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with one unit o...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 50 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"