概述

  • 产品名称Anti-IL13抗体
    参阅全部 IL13 一抗
  • 描述
    兔多克隆抗体to IL13
  • 经测试应用适用于: WB, ELISA, Neutralising, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hIL-13 (human Interleukin-13)

  • 阳性对照
    • IHC-P: human testis tissue sections

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab9576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-13 (assuming 100 µl/well antibody solution).
Neutralising Use a concentration of 0.12 - 0.13 µg/ml. for one-half maximal inhibition [ND50] of the biological activity of hIL-13 (2.0 ng/ml).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 疾病相关Allergic rhinitis
  • 序列相似性Belongs to the IL-4/IL-13 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

Anti-IL13 antibody 图像

  • All lanes : Anti-IL13 antibody (ab9576) at 1/3000 dilution

    Lane 1 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.2 µg
    Lane 2 : E. coli expressed human recombinant protein IL13 from supplier 1 at 0.3 µg
    Lane 3 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.2 µg
    Lane 4 : E. coli expressed human recombinant protein IL13 from supplier 2 at 0.3 µg
    Lane 5 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.2 µg
    Lane 6 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.3 µg
    Lane 7 : E. coli expressed human recombinant protein IL13 from supplier 3 at 0.4 µg

    Secondary
    HRP conjugated goat anti-rabbit at 1/2000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 26 kDa (possible dimer).

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Miss Fenny Chong

    See Abreview

  • IHC image of IL13 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9576, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-IL13 antibody (ab9576)参考文献

ab9576 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lung)
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Ventana Protease 1
Permeabilization No
Specification Lung
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (E.coli expressed commercial protein)
Loading amount 0.3 µg
Specification E.coli expressed commercial protein
Gel Running Conditions Non-reduced Denaturing (15%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Miss. Fenny Chong

Verified customer

提交于 Mar 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"