概述

  • 产品名称
  • 描述
    兔多克隆抗体to IL13
  • 经测试应用
    适用于: Indirect ELISA, Neutralising, Sandwich ELISA, WBmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Mouse
  • 免疫原

    Highly pure (>98%) recombinant Mouse IL13.

性能

应用

Our Abpromise guarantee covers the use of ab109815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Mouse IL13.
Neutralising Use a concentration of 0.08 - 0.1 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL13 (10.0 ng/ml)
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL13 (Biotin) (ab83162). Using ab83162 as the detection antibody and ab109815 as the capture antibody, the detection limit for recombinant Mouse IL13 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Mouse IL13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

靶标

  • 功能
    Cytokine (PubMed:8096327, PubMed:8097324). Inhibits inflammatory cytokine production (PubMed:8096327). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed:8096327). May be critical in regulating inflammatory and immune responses (PubMed:8096327, PubMed:8097324). Positively regulates IL31RA expression in macrophages.
  • 疾病相关
    Allergic rhinitis
  • 序列相似性
    Belongs to the IL-4/IL-13 family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Allergic rhinitis antibody
    • ALRH antibody
    • BHR1 antibody
    • Bronchial hyperresponsiveness 1 (bronchial asthma) antibody
    • IL 13 antibody
    • IL-13 antibody
    • Il13 antibody
    • IL13_HUMAN antibody
    • Interleukin 13 antibody
    • Interleukin-13 antibody
    • MGC116786 antibody
    • MGC116788 antibody
    • MGC116789 antibody
    • NC30 antibody
    • P600 antibody
    see all

实验方案

文献

This product has been referenced in:
  • McSorley HJ  et al. Expansion of Foxp3+ regulatory T cells in mice infected with the filarial parasite Brugia malayi. J Immunol 181:6456-66 (2008). Read more (PubMed: 18941236) »

See 1 Publication for this product

客户评价及客户问答

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Gel Running Conditions
Reduced Denaturing (15% gel)
Loading amount
5 µg
Treatment
25 ng/ml LPS for 6, 12, 24, 36 and 48h
Specification
Raw 264.7
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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提交于 Dec 19 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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