Anti-IL11RA抗体[EPR5446] (ab125015)


  • 产品名称Anti-IL11RA抗体[EPR5446]
    参阅全部 IL11RA 一抗
  • 描述
    兔单克隆抗体[EPR5446] to IL11RA
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide, corresponding to residues in Human IL11RA.

  • 阳性对照
    • 293T, Saos2, A673, PC3, HuT78, K562, and TF1 cell lysates; Human kidney tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-IL11RA antibody (HRP) [EPR5446] (ab202923)

    Anti-IL11RA antibody (Biotin) [EPR5446] (ab202924)



Our Abpromise guarantee covers the use of ab125015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and/or differentiation of skeletogenic progenitor or other mesenchymal cells.
    • 组织特异性Expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. Also expressed in normal and malignant prostate epithelial cell lines. Expression levels are increased in prostate carcinoma.
    • 序列相似性Belongs to the type I cytokine receptor family. Type 3 subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CRSDA antibody
      • hCG_2011440 antibody
      • I11RA_HUMAN antibody
      • IL-11 receptor subunit alpha antibody
      • IL-11R subunit alpha antibody
      • IL-11R-alpha antibody
      • IL-11RA antibody
      • IL11RA antibody
      • Interleukin 11 receptor alpha antibody
      • Interleukin 11 receptor alpha chain antibody
      • Interleukin-11 receptor subunit alpha antibody
      • MGC2146 antibody
      see all

    Anti-IL11RA antibody [EPR5446] 图像

    • Overlay histogram showing K562 cells stained with ab125015 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab125015, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • All lanes : Anti-IL11RA antibody [EPR5446] (ab125015) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Saos2 lysate
      Lane 3 : A673 lysate
      Lane 4 : PC3 lysate
      Lane 5 : Hut78 lysate
      Lane 6 : K562 lysate
      Lane 7 : TF1 lysate

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 45 kDa
      Observed band size : 45 kDa
    • ab125015 at 1/500 staining IL11RA in paraffin embedded Human Kidney tissue by immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-IL11RA antibody [EPR5446] (ab125015)参考文献

    ab125015 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply.

    Indeed, while this product is tested and guaranteed to work in Flow Cytometry, as the epitope is cytoplasmic, cells will need to be permeablized.

    I hope this information is helpful to you. Please do n...

    Read More

    Thank you for contacting us.

    The exact immunogen information for this product is considered proprietary and as such I am unable to share the sequence with you. I can however let you know that the immunogen used to create this product did c...

    Read More

    Thank you for contacting us.

    For the WB image containing various cell line lysates, 10 ug of protein was loaded.
    5% non fat milk in TBST was used for blocking for 1 hr, Primary antibodu was used at a dilution of 1/1000 in 5%BSA/TBST over...

    Read More