概述

性能

应用

Our Abpromise guarantee covers the use of ab76589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 21 kDa).

靶标

  • 功能
    Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production.
  • 序列相似性
    Belongs to the IL-6 superfamily.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adipogenesis inhibitory factor antibody
    • AGIF antibody
    • IL 11 antibody
    • IL-11 antibody
    • Il11 antibody
    • IL11_HUMAN antibody
    • Interleukin 11 antibody
    • Interleukin-11 antibody
    • Oprelvekin antibody
    see all

图片

  • Anti-IL11 antibody (ab76589) at 1 µg/ml + Human Plasma Total Protein Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 21 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • Anti-IL11 antibody (ab76589) at 1 µg/ml + Recombinant human IL11 protein (ab73620) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

实验方案

文献

This product has been referenced in:
  • Marthandan S  et al. Similarities in Gene Expression Profiles during In Vitro Aging of Primary Human Embryonic Lung and Foreskin Fibroblasts. Biomed Res Int 2015:731938 (2015). WB . Read more (PubMed: 26339636) »
  • Jiang Y  et al. Downregulation of SPARC expression inhibits the invasion of human trophoblast cells in vitro. PLoS One 8:e69079 (2013). WB . Read more (PubMed: 23935929) »

See all 2 Publications for this product

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