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Our Abpromise guarantee covers the use of ab109813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IL11.|
|Neutralising||Use a concentration of 0.02 - 0.05 µg/ml. to yield one-half maximal inhibition [ND50] of the biological activity of IL11 (1.5 ng/ml)|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to IL11 (Biotin) (ab83120). Using ab83120 as the detection antibody and ab109813 as the capture antibody, the detection limit for recombinant Human IL11 is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 21 kDa. The detection limit for recombinant Human IL11 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
ab109813 has not yet been referenced specifically in any publications.