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Recombinant full length protein (Mouse)
ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.
Our Abpromise guarantee covers the use of ab33471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 17 kDa. We recommend incubating the membrane overnight in primary antibody.|
|Flow Cyt||1/10 - 1/100.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
|ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"