Anti-IL10抗体[JES5-2A5] (ab33471)

概述

  • 产品名称Anti-IL10抗体[JES5-2A5]
    参阅全部 IL10 一抗
  • 描述
    大鼠单克隆抗体[JES5-2A5] to IL10
  • 经测试应用适用于: WB, Flow Cyt, IHC-Fr, ELISAmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Recombinant full length protein (Mouse)

  • 常规说明


    ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Primary antibody说明ab33471 recognises mouse Interleukin 10 (IL 10). IL 10 is expressed by Ly 1 (B 1) B cells that express CD5 (Ly 1) and a subset of lectin stimulated T cells. Clone JES 5-2A5 is reported to neutralise the activity of murine IL 10.
  • 克隆单克隆
  • 克隆编号JES5-2A5
  • 骨髓瘤P3x63-Ag8.653
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab33471 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa. We recommend incubating the membrane overnight in primary antibody.
Flow Cyt 1/10 - 1/100.

Use 10ul of the suggested working dilution to label 106 cells in 100ul.
ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/100.
ELISA Use at an assay dependent concentration.

靶标

  • 功能Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
  • 组织特异性Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
  • 序列相似性Belongs to the IL-10 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CSIF antibody
    • Cytokine synthesis inhibitory factor antibody
    • GVHDS antibody
    • IL 10 antibody
    • IL-10 antibody
    • IL10 antibody
    • IL10_HUMAN antibody
    • IL10A antibody
    • Interleukin 10 antibody
    • Interleukin-10 antibody
    • MGC126450 antibody
    • MGC126451 antibody
    • T-cell growth inhibitory factor antibody
    • TGIF antibody
    see all

Anti-IL10 antibody [JES5-2A5] 图像

Anti-IL10 antibody [JES5-2A5] (ab33471)参考文献

This product has been referenced in:
  • Yao L  et al. Notch-1 Signaling Regulates Microglia Activation via NF-?B Pathway after Hypoxic Exposure In Vivo and In Vitro. PLoS One 8:e78439 (2013). WB ; Mouse . Read more (PubMed: 24223152) »
  • Fyhrquist N  et al. Foxp3+ cells control Th2 responses in a murine model of atopic dermatitis. J Invest Dermatol 132:1672-80 (2012). Mouse . Read more (PubMed: 22402436) »

See all 8 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain)
Permeabilization Yes - 0.1 M PBS with 3% Triton X
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Jun 25 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native)
Sample Mouse Tissue lysate - whole (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 13 2014

Thank you for contacting us.

I have found some information regarding the anti-IL-10 antibodies which we spoke of. To begin, I have found that Mouse IL-10 and Human IL-10 have only a 73% homology which is especially poor in the region around ...

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Thank you for contacting us.

We haven't tested this antibody in paraffin embedded tissue sections (IHC-P), only frozen sections from mouse. However, IL10 is produced and secreted by a variety of cell lines, including T-cells, macrophages, ma...

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We typically load 10 - 20 nanograms of purified recombinant protein in gels for western blots. I have contacted the laboratory to find out the exact amount that was used for the blot on the datasheet but I expect it will be within that range.

Thank you for contacting us.

We recommend ab18412.

Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply and for sending your protocol information and image. We do guarantee ab33471 to work in Western blot with mouse lysates, so we are happy to issue you a refund for this purchase. I have requested the refund to our accoun...

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According to our records, ab33471 was proving difficult to use in IHC and we were in contact in order to help resolve the issue. Looking at our correspondence, it appears that we are awaiting more details in order for us to resolve the problem. If the ...

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Thank you for contacting us, and I am sorry that this antibody has not produced expected results. We are happy to offer a refund if a product does not work in a tested species and application within 6 months of purchase. Do you have your order number? ...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"