The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.25 - 1 µg/ml.
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Can be paired for ELISA with Rabbit polyclonal to IL10 (ab9611) WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 21 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.