产品名称 Anti-IL10抗体[B-S10] 参阅全部 IL10 一抗
小鼠单克隆抗体[B-S10] to IL10
特异性 This antibody recognizes both natural and recombinant IL10.
Blocking, ELISA more details
Recombinant human IL10.
存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 Preservative: None
Concentration information loading...
纯度 Ion Exchange Chromatography
纯化说明 This antibody is sterile filtered through 0.22 µm and treated to remove endotoxins. It is carrier and preservative free.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明 BL: Use at an assay dependent dilution.
The antibody blocks IL10 induced proliferation on MC/9 cell line.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能 Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
组织特异性 Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
序列相似性 Belongs to the IL-10 family.
Information by UniProt
Cytokine synthesis inhibitory factor antibody
Anti-IL10 antibody [B-S10] 图像
ELISA - Anti-IL10 antibody [B-S10] - Azide free (ab47222) Image courtesy of an anonymous Abreview.
We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10. ab47222 used at a 1/2000 dilution.
Anti-IL10 antibody [B-S10] (ab47222)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"