Anti-IL10抗体[B-S10] (ab47222)

概述

  • 产品名称
    Anti-IL10抗体[B-S10]
    参阅全部 IL10 一抗
  • 描述
    小鼠单克隆抗体[B-S10] to IL10
  • 特异性
    This antibody recognizes both natural and recombinant IL10.
  • 经测试应用
    适用于: Blocking, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human IL10.

性能

应用

Our Abpromise guarantee covers the use of ab47222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Blocking
ELISA
  • 应用说明
    BL: Use at an assay dependent dilution.
    The antibody blocks IL10 induced proliferation on MC/9 cell line.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
    • 组织特异性
      Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
    • 序列相似性
      Belongs to the IL-10 family.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CSIF antibody
      • Cytokine synthesis inhibitory factor antibody
      • GVHDS antibody
      • IL 10 antibody
      • IL-10 antibody
      • IL10 antibody
      • IL10_HUMAN antibody
      • IL10A antibody
      • Interleukin 10 antibody
      • Interleukin-10 antibody
      • MGC126450 antibody
      • MGC126451 antibody
      • T-cell growth inhibitory factor antibody
      • TGIF antibody
      see all

    图片

    • We examined the effect of anti IL-10 to study the regulatory role of IL-10 and the interaction of IL-10 with its receptor. Measurement of neutralizing activity of anti IL-10 by TNFa-ELISA. IL-10 decreased the LPS-induced production of TNFa by Monocytes and recombinant IL-10 is neutralized by increasing concentrations of anti IL-10 antibody. The TNFa production showed a dependence on antibody concentration of anti IL-10.
      ab47222 used at a 1/2000 dilution.

      See Abreview

    文献

    ab47222 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    ELISA
    Type
    Direct
    Sample
    Human Cell (Monocytes)
    Specification
    Monocytes
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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