The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
Neut: Use at an assay dependent dilution. 600pg of ab22771 neutralised 50% of the activity of 1pg of IL10. Removal of sodium azide is recommended prior to functional assays.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Tsuji H et al. Xenografted human amniotic membrane-derived mesenchymal stem cells are immunologically tolerated and transdifferentiated into cardiomyocytes. Circ Res106:1613-23 (2010).
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