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Our Abpromise guarantee covers the use of ab9969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Can be blocked with Recombinant rat IL10 protein (ab9970).|
|ELISA||Use a concentration of 0.5 µg/ml.|
|Neutralising||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of rIL-10 (30.0 ng/ml), a concentration of 0.45 - 0.8 µg/ml of this antibody is required.|
|ICC/IF||Use at an assay dependent concentration.|