Anti-IL1 beta抗体(HRP) (ab106015)

概述

  • 产品名称Anti-IL1 beta抗体(HRP)
    参阅全部 IL1 beta 一抗
  • 描述
    兔多克隆抗体to IL1 beta (HRP)
  • 偶联物HRP
  • 特异性This antibody recognises mature Human IL1B. It does not recognize Human IL1A. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31 kDa IL1B containing samples but will primarily detect all of the 17 kDa mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31 kDa IL1B precursor molecule. Denatured 31 kDa precursor IL1B will be recognized by this antibody but often migrates as a 35 kDa band. In immunoblots, depending on the number of cells, the antibody detects the 17 kDa band in supernatants as well as a 35 kDa band representing the 31 kDa IL1B precursor in lysates.
  • 经测试应用适用于: IHC-Fr, WB, IP, ELISA, IHC-P, RIA, Neutralising, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human, Non Human Primates
  • 免疫原

    Recombinant Human IL1B produced in E. coli

  • 阳性对照
    • Peripheral blood mononuclear cells stimulated with LPS

性能

应用

Our Abpromise guarantee covers the use of ab106015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
IP Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000. This antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody.
IHC-P 1/500 - 1/2500.
RIA Use at an assay dependent dilution.
Neutralising Use at an assay dependent dilution. It is recommended to incubate the sample with a dilution of the antibody for at least 4 hours.
Flow Cyt Use at an assay dependent dilution. Caution should be exhibited as the F(c) domain of the antibody may interact with cells non-specifically.

靶标

  • 功能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
  • 组织特异性Expressed in activated monocytes/macrophages (at protein level).
  • 序列相似性Belongs to the IL-1 family.
  • 翻译后修饰Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
  • 细胞定位Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Catabolin antibody
    • H1 antibody
    • IL 1 antibody
    • IL 1 beta antibody
    • IL-1 beta antibody
    • IL1 BETA antibody
    • IL1B antibody
    • IL1B_HUMAN antibody
    • IL1F2 antibody
    • Interleukin 1 beta antibody
    • Interleukin-1 beta antibody
    • OAF antibody
    • OTTHUMP00000162031 antibody
    • Preinterleukin 1 beta antibody
    • Pro interleukin 1 beta antibody
    see all

Anti-IL1 beta antibody (HRP) (ab106015)参考文献

ab106015 has not yet been referenced specifically in any publications.

Product Wall


We have 3 IL-1B antibodies that have been tested in IHC-P and WB with human samples and that as well state to recognize the active/mature form:

ab2105, ab34837, ab106015
http://www.abcam.com/il1-beta-antibody-ab2105.html
http://ww...

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Thank you for contacting Abcam.

For ab106015, as it already has HRP conjugated to it, you will not need to use a HRP tagged secondary antibody with it. You would incubate the primary antibody as normal, wash the membrane and then add your ECL...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"